CCAR2 Polyclonal antibody

Name: CCAR2 Polyclonal antibody
Brand: Proteintech
SKU: 22638-1-AP

CCAR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22638-1-AP

Synonyms

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 22638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22638-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22638-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 22638-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22638-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22638-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 22638-1-AP
IP result of anti-CCAR2 (IP:22638-1-AP, 4ug; Detection:22638-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human colon cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.
IHC staining of human breast cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.
IHC staining of human pancreas cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.
IHC staining of human pancreas cancer using 22638-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.
IF Staining of HepG2 using 22638-1-AP
Immunofluorescent analysis of HepG2 cells, using KIAA1967 antibody 22638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 22638-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

22638-1-AP targets CCAR2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CCAR2 fusion protein Ag18374 相同性解析による交差性が予測される生物種
Full Name	KIAA1967
Calculated molecular weight	923 aa, 103 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC018269
Gene symbol	CCAR2
Gene ID (NCBI)	57805
RRID	AB_2879138
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CCAR2 antibody 22638-1-AP	Download protocol
IHC protocol for CCAR2 antibody 22638-1-AP	Download protocol
IF protocol for CCAR2 antibody 22638-1-AP	Download protocol
IP protocol for CCAR2 antibody 22638-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	CoIP,RIP	Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. Authors - Ruibing Chen View Article
mouse	WB, IF	Biochem Cell Biol Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1. Authors - Farshad Niri View Article
human	WB,IHC,IP	Elife Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression Authors - Qiangqiang Liu View Article
mouse	IF	Br J Pharmacol AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease Authors - Fengjiao Sun View Article
human	WB	Redox Biol SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3 Authors - Xi Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCAR2 Polyclonal antibody

CCAR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 22638-1-AP

WB analysis of mouse brain using 22638-1-AP

WB analysis of MCF-7 using 22638-1-AP

WB analysis of rat brain using 22638-1-AP

WB analysis of HeLa using 22638-1-AP

WB analysis of HEK-293 using 22638-1-AP

IP experiment of mouse brain using 22638-1-AP

IHC staining of human colon cancer using 22638-1-AP

IHC staining of human colon cancer using 22638-1-AP

IHC staining of human breast cancer using 22638-1-AP

IHC staining of human breast cancer using 22638-1-AP

IHC staining of human pancreas cancer using 22638-1-AP

IHC staining of human pancreas cancer using 22638-1-AP

IF Staining of HepG2 using 22638-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.

Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.

Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression

AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease

SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3