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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DBF4 Polyclonal antibody

DBF4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13076-1-AP

Synonyms

Activator of S phase kinase, ASK, CHIF, Chiffon homolog A, DBF4, DBF4 homolog (S. cerevisiae), DBF4A, Protein DBF4 homolog A, ZDBF1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using DBF4 Polyclonal antibody (13076-1-AP)

    WB analysis of NIH/3T3 using 13076-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP (DBF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DBF4 Polyclonal antibody (13076-1-AP)

    WB analysis of NIH/3T3 using 13076-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP (DBF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inNIH/3T3 cells, Jurkat cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13076-1-AP targets DBF4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBF4 fusion protein Ag3771 相同性解析による交差性が予測される生物種
Full Name DBF4 homolog (S. cerevisiae)
Calculated molecular weight77 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC036045
Gene symbol DBF4
Gene ID (NCBI) 10926
RRIDAB_10638788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DBF4 antibody 13076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols