Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | PC-3 cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26214-1-AP targets DBF4B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DBF4B fusion protein Ag23889 相同性解析による交差性が予測される生物種 |
| Full Name | DBF4 homolog B (S. cerevisiae) |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC016158 |
| Gene Symbol | DBF4B |
| Gene ID (NCBI) | 80174 |
| RRID | AB_3669528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cell-cycle progression is driven by orderly activation of many kinases including the DBF4/DBF4B-dependent S-phase promoting kinase Cdc7. Binding of Cdc7 to its subunit DBF4 or DBF4B (also known as DRF1/ASKL1) is thought to be a key regulatory event that controls Cdc7 activity in human cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DBF4B antibody 26214-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |