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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DBH Polyclonal antibody

DBH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10777-1-AP

Synonyms

Soluble dopamine beta-hydroxylase, EC:1.14.17.1, Dopamine beta-monooxygenase, Dopamine beta-hydroxylase, DBM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DBH Polyclonal antibody (10777-1-AP)

    WB analysis using 10777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using DBH Polyclonal antibody (10777-1-AP)

    WB analysis of SH-SY5Y using 10777-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBH Polyclonal antibody (10777-1-AP)

    IHC staining of mouse brain using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBH Polyclonal antibody (10777-1-AP)

    IHC staining of mouse brain using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DBH Polyclonal antibody (10777-1-AP)

    IHC staining of human liver cancer using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DBH Polyclonal antibody (10777-1-AP)

    IHC staining of human liver cancer using 10777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DBH Polyclonal antibody (10777-1-AP)

    IF Staining of HepG2 using 10777-1-AP

    Immunofluorescent analysis of HepG2 cells using 10777-1-AP (DBH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DBH Polyclonal antibody (10777-1-AP)

    IF Staining of HeLa using 10777-1-AP

    Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using DBH Polyclonal antibody (10777-1-AP)

    FC experiment of SH-SY5Y using 10777-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inSH-SY5Y cells, NIH/3T3 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10777-1-AP targets DBH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBH fusion protein Ag1196 相同性解析による交差性が予測される生物種
Full Name DBH
Calculated molecular weight64.8 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC017174
Gene symbol DBH
Gene ID (NCBI) 1621
RRIDAB_2277037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).

Protocols

Product Specific Protocols
WB protocol for DBH antibody 10777-1-APDownload protocol
IHC protocol for DBH antibody 10777-1-APDownload protocol
IF protocol for DBH antibody 10777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.

Authors - Shin Takasawa
View Article
humanWB

Peptides

Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid.

Authors - Yuan-Jun Qin
View Article