Drebrin Polyclonal antibody

Name: Drebrin Polyclonal antibody
Brand: Proteintech
SKU: 10260-1-AP

Drebrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10260-1-AP

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 10260-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10260-1-AP (DBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10260-1-AP (Drebrin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10260-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10260-1-AP (Drebrin Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10260-1-AP
IP result of anti-Drebrin (IP:10260-1-AP, 4ug; Detection:10260-1-AP 1:2000) with K-562 cells lysate 1200ug.
IHC staining of mouse testis using 10260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10260-1-AP (Drebrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Drebrin antibody (10260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Drebrin antibody (10260-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10260-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10260-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10260-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10260-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10260-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, Neuro-2a cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 18 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

10260-1-AP targets Drebrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Drebrin fusion protein Ag0371 相同性解析による交差性が予測される生物種
Full Name	drebrin 1
Calculated molecular weight	71 kDa
Observed molecular weight	100-130 kDa
GenBank accession number	BC000283
Gene symbol	Drebrin
Gene ID (NCBI)	1627
RRID	AB_2230301
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 10260-1-AP	Download protocol
IHC protocol for Drebrin antibody 10260-1-AP	Download protocol
IF protocol for Drebrin antibody 10260-1-AP	Download protocol
IP protocol for Drebrin antibody 10260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration Authors - Shifaan Thowfeequ View Article
mouse	IF	PLoS Genet N-wasp is required for structural integrity of the blood-testis barrier. Authors - Xiang Xiao View Article
human	IF	Int J Mol Sci Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery Authors - Paloma Alvarez-Suarez View Article
human	WB	Oncogenesis Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels Authors - Jin Wang View Article
human	WB	Biol Direct Glucose deprivation-induced disulfidptosis in human nucleus pulposus cells: a novel pathological mechanism of intervertebral disc degeneration Authors - Shaobo Wu View Article
mouse,rat	WB	Mol Neurobiol L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons. Authors - Longjian Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10260-1-AP

WB analysis using 10260-1-AP

WB analysis of K-562 using 10260-1-AP

IP experiment of K-562 using 10260-1-AP

IHC staining of mouse testis using 10260-1-AP

IHC staining of mouse testis using 10260-1-AP

IF Staining of HeLa using 10260-1-AP

IF Staining of HepG2 using 10260-1-AP

IF Staining of HeLa using 10260-1-AP

IF Staining of HepG2 using 10260-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration

N-wasp is required for structural integrity of the blood-testis barrier.

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Tumor suppressor BAP1 suppresses disulfidptosis through the regulation of SLC7A11 and NADPH levels

Glucose deprivation-induced disulfidptosis in human nucleus pulposus cells: a novel pathological mechanism of intervertebral disc degeneration

L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.