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KD/KO Validated

HIP55 Polyclonal antibody

Name: HIP55 Polyclonal antibody
Brand: Proteintech
SKU: 13015-1-AP

HIP55 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13015-1-AP

Synonyms

DBNL, HPK1-interacting protein of 55 kDa, Drebrin-like protein, Drebrin-F, Cervical mucin-associated protein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse brain using 13015-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 13015-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13015-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13015-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13015-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13015-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13015-1-AP
IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug.
IHC staining of human breast cancer using 13015-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13015-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13015-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 13015-1-AP
Immunofluorescent analysis of HepG2 cells, using DBNL antibody 13015-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).
FC experiment of HepG2 using 13015-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 13015-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, human heart tissue, Jurkat cells, K-562 cells, mouse heart tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

13015-1-AP targets HIP55 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HIP55 fusion protein Ag4078 相同性解析による交差性が予測される生物種
Full Name	drebrin-like
Calculated molecular weight	430 aa, 48 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC031687
Gene symbol	HIP55
Gene ID (NCBI)	28988
RRID	AB_2230306
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

Protocols

Product Specific Protocols
WB protocol for HIP55 antibody 13015-1-AP	Download protocol
IHC protocol for HIP55 antibody 13015-1-AP	Download protocol
IF protocol for HIP55 antibody 13015-1-AP	Download protocol
IP protocol for HIP55 antibody 13015-1-AP	Download protocol
FC protocol for HIP55 antibody 13015-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IP	Acta Pharmacol Sin The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation. Authors - Yang Sun KD Validated View Article
mouse	WB,IF,IP	J Neurosci Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex. Authors - Seika Inoue View Article
		J Cell Sci VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. Authors - Thomas Daubon View Article
mouse	WB	J Microbiol Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model. Authors - Yun Misun M View Article
human	IF	Nature A cross-species proteomic map reveals neoteny of human synapse development Authors - Li Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIP55 Polyclonal antibody

HIP55 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of mouse brain using 13015-1-AP

WB analysis of mouse heart using 13015-1-AP

WB analysis of HeLa using 13015-1-AP

WB analysis of human heart using 13015-1-AP

WB analysis of K-562 using 13015-1-AP

WB analysis of Jurkat using 13015-1-AP

IP experiment of mouse brain using 13015-1-AP

IHC staining of human breast cancer using 13015-1-AP

IHC staining of human breast cancer using 13015-1-AP

IF Staining of HepG2 using 13015-1-AP

IF Staining of HepG2 using 13015-1-AP

FC experiment of HepG2 using 13015-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.

Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex.

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells.

Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model.

A cross-species proteomic map reveals neoteny of human synapse development