Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue, HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
16019-1-AP targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DBR1 fusion protein Ag8830 相同性解析による交差性が予測される生物種 |
| Full Name | debranching enzyme homolog 1 (S. cerevisiae) |
| Calculated molecular weight | 544 aa, 62 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC009472 |
| Gene Symbol | DBR1 |
| Gene ID (NCBI) | 51163 |
| RRID | AB_2230316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IHC protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IF protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IP protocol for DBR1 antibody 16019-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.
| ||
Mol Cell Proteomics Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition. | ||
PLoS One Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus. | ||
Int J Mol Sci Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein. |