Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of human liver using 13168-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 13168-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 13168-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13168-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of U2OS using 13168-1-AP
IP result of anti-DCBLD2 (IP:13168-1-AP, 4ug; Detection:13168-1-AP 1:1000) with U2OS cells lysate 440 ug.
IHC staining of human intrahepatic cholangiocarcinoma using 13168-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13168-1-AP (DCBLD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 13168-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13168-1-AP (DCBLD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

DCBLD2 Polyclonal antibody

DCBLD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13168-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

ESDN, Endothelial and smooth muscle cell-derived neuropilin-like protein, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, CLCP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MKN-45 cells, human liver tissue, human brain tissue, SW 1990 cells, U2OS cells
Positive IP detected in	U2OS cells
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

13168-1-AP targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DCBLD2 fusion protein Ag3821 相同性解析による交差性が予測される生物種
Full Name	discoidin, CUB and LCCL domain containing 2
Calculated molecular weight	775 aa, 89 kDa
Observed molecular weight	93 kDa, 127 kDa
GenBank accession number	BC029658
Gene Symbol	DCBLD2
Gene ID (NCBI)	131566
RRID	AB_2292662
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96PD2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

Protocols

Product Specific Protocols
WB protocol for DCBLD2 antibody 13168-1-AP	Download protocol
IHC protocol for DCBLD2 antibody 13168-1-AP	Download protocol
IP protocol for DCBLD2 antibody 13168-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Environ Toxicol Comprehensive analyses and experimental verification of NETs and an EMT gene signature for prognostic prediction, immunotherapy, and chemotherapy in pancreatic adenocarcinoma Authors - Jing Zhang KD Validated View Article
human	WB,IHC	Am J Cancer Res In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer Authors - Qiankun Zhu View Article
mouse	WB	Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin Authors - Yuqi Xin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human liver using 13168-1-AP

WB analysis using 13168-1-AP

WB analysis using 13168-1-AP

WB analysis of human brain using 13168-1-AP

IP experiment of U2OS using 13168-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 13168-1-AP