- Featured Product
- KD/KO Validated
DCBLD2 Polyclonal antibody
DCBLD2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13168-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MKN-45 cells, human liver tissue, human brain tissue, SW 1990 cells, U2OS cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
13168-1-AP targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DCBLD2 fusion protein Ag3821 相同性解析による交差性が予測される生物種 |
| Full Name | discoidin, CUB and LCCL domain containing 2 |
| Calculated molecular weight | 775 aa, 89 kDa |
| Observed molecular weight | 93 kDa, 127 kDa |
| GenBank accession number | BC029658 |
| Gene symbol | DCBLD2 |
| Gene ID (NCBI) | 131566 |
| RRID | AB_2292662 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| IHC protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| IP protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Toxicol Comprehensive analyses and experimental verification of NETs and an EMT gene signature for prognostic prediction, immunotherapy, and chemotherapy in pancreatic adenocarcinoma
| ||
Am J Cancer Res In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer | ||
Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin |
