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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCBLD2 Polyclonal antibody (30245-1-AP)

    WB analysis using 30245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30245-1-AP (DCBLD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DCBLD2 Polyclonal antibody (30245-1-AP)

    IHC staining of human stomach cancer using 30245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DCBLD2 Polyclonal antibody (30245-1-AP)

    IHC staining of human stomach cancer using 30245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DCBLD2 Polyclonal antibody

DCBLD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30245-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ESDN, Endothelial and smooth muscle cell-derived neuropilin-like protein, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, CLCP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30245-1-AP targets DCBLD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCBLD2 fusion protein Ag32823 相同性解析による交差性が予測される生物種
Full Name discoidin, CUB and LCCL domain containing 2
Calculated molecular weight 775 aa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberBC029658
Gene Symbol DCBLD2
Gene ID (NCBI) 131566
RRIDAB_3086277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

Protocols

Product Specific Protocols
WB protocol for DCBLD2 antibody 30245-1-APDownload protocol
IHC protocol for DCBLD2 antibody 30245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols