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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCI Polyclonal antibody

DCI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11535-1-AP

Synonyms

ECI1, EC:5.3.3.8, Dodecenoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCI Polyclonal antibody (11535-1-AP)

    WB analysis using 11535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DCI Polyclonal antibody (11535-1-AP)

    WB analysis using 11535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human lung using 11535-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human brain using 11535-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human kidney using 11535-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of BxPC-3 using 11535-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DCI Polyclonal antibody (11535-1-AP)

    IHC staining of human prostate cancer using 11535-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using DCI Polyclonal antibody (11535-1-AP)

    IF Staining of BxPC-3 using 11535-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DCI Polyclonal antibody (11535-1-AP)

    IF Staining of HEK-293 using 11535-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inCOLO 320 cells, human lung tissue, human brain tissue, human kidney tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCI fusion protein Ag2094 相同性解析による交差性が予測される生物種
Full Name dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Calculated molecular weight 302 aa, 33 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC020228
Gene symbol DCI
Gene ID (NCBI) 1632
RRIDAB_2089906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

Protocols

Product Specific Protocols
WB protocol for DCI antibody 11535-1-APDownload protocol
IHC protocol for DCI antibody 11535-1-APDownload protocol
IF protocol for DCI antibody 11535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols