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KD/KO Validated

DCK Polyclonal antibody

Name: DCK Polyclonal antibody
Brand: Proteintech
SKU: 17758-1-AP

DCK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17758-1-AP

Synonyms

DCK, deoxycytidine kinase

Validation Data Gallery

Filter:

AllWB

WB analysis using 17758-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17758-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17758-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17758-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17758-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Cat No. 17758-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below

Product Information

17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DCK fusion protein Ag12123 相同性解析による交差性が予測される生物種
Full Name	deoxycytidine kinase
Calculated molecular weight	260 aa, 31 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC103764
Gene symbol	DCK
Gene ID (NCBI)	1633
RRID	AB_2261476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).

Protocols

Product Specific Protocols
WB protocol for DCK antibody 17758-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer. Authors - Yaoyi Xiong View Article
human	WB	Am J Cancer Res PMID: 32368394 Authors - Zeng Ye View Article
human	WB	Int J Cancer Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism. Authors - Nao Yoshida-Sakai View Article
human	WB	Front Oncol m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer. Authors - Congjun Zhang View Article
human	WB	Invest New Drugs Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma. Authors - Irene Dell'Anno View Article
human	WB	Sci Rep Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia. Authors - Susan K Rathe KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCK Polyclonal antibody

DCK Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis using 17758-1-AP

WB analysis of Jurkat using 17758-1-AP

WB analysis of HeLa using 17758-1-AP

WB analysis of MCF-7 using 17758-1-AP

WB analysis of HepG2 using 17758-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer.

PMID: 32368394

Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.

m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.

Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma.

Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia.