DCLK1 Monoclonal antibody

DCLK1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3C12C1

Cat no : 68234-1-Ig

Synonyms

Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1


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Tested Applications

Positive WB detected inpig brain tissue, fetal human brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68234-1-Ig targets DCLK1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DCLK1 fusion protein Ag17110 相同性解析による交差性が予測される生物種
Full Name doublecortin-like kinase 1
Calculated molecular weight 729 aa, 81 kDa
Observed molecular weight46 kDa, 82 kDa
GenBank accession numberBC152456
Gene symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

Protocols

Product Specific Protocols
WB protocol for DCLK1 antibody 68234-1-IgDownload protocol
IHC protocol for DCLK1 antibody 68234-1-IgDownload protocol
IF protocol for DCLK1 antibody 68234-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cerys (Verified Customer) (08-14-2024)

Very easy to use and gives a clear signal.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 in 500
  • Cell Tissue Type: Murine Small Intestine, 1%PFA, OCT
DCLK1 Antibody Immunohistochemistry validation (1 in 500 dilution) in Murine Small Intestine, 1%PFA, OCT (Cat no:68234-1-Ig)