DCP1A Polyclonal antibody

Name: DCP1A Polyclonal antibody
Brand: Proteintech
SKU: 22373-1-AP
Rating: 5.00 (2 reviews)

DCP1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 22373-1-AP

Synonyms

Smad4-interacting transcriptional co-activator, Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 22373-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22373-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 22373-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 22373-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 22373-1-AP
IP result of anti-DCP1A (IP:22373-1-AP, 4ug; Detection:22373-1-AP 1:3000) with mouse brain tissue lysate 3200ug.
IHC staining of mouse lung using 22373-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse lung using 22373-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 22373-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 22373-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 22373-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DCP1A (22373-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 22373-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, mouse lung tissue, Jurkat cells, MCF-7 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

22373-1-AP targets DCP1A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DCP1A fusion protein Ag18027 相同性解析による交差性が予測される生物種
Full Name	DCP1 decapping enzyme homolog A (S. cerevisiae)
Calculated molecular weight	582 aa, 63 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC007439
Gene symbol	DCP1A
Gene ID (NCBI)	55802
RRID	AB_2879092
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Protocols

Product Specific Protocols
WB protocol for DCP1A antibody 22373-1-AP	Download protocol
IHC protocol for DCP1A antibody 22373-1-AP	Download protocol
IF protocol for DCP1A antibody 22373-1-AP	Download protocol
IP protocol for DCP1A antibody 22373-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	FEBS Lett Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells. Authors - Dongxu Jiang View Article
human	WB,IF	Cell Rep DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling Authors - Ke Zhang View Article
mouse	IF,IP	Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation Authors - Nicolas Curdy View Article
human	WB	J Thorac Dis LncRNA HOXD-AS2 regulates miR-3681-5p/DCP1A axis to promote the progression of non-small cell lung cancer Authors - Yuanyuan Zhang View Article
human	IF,WB	J Cell Biol DDX6 modulates P-body and stress granule assembly, composition, and docking Authors - Nina Ripin View Article
human	WB	J Proteome Res Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A Authors - Qihang Peng View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Laurens (Verified Customer) (02-02-2024)	Works well for ICC with bright stain and no background, shown with green channel in image. Applications: Immunofluorescence Primary Antibody Dilution: 1:500 Cell Tissue Type: A549 cells, U2OS cells, HEK293 cells DCP1A Antibody Immunofluorescence validation (1:500 dilution) in A549 cells, U2OS cells, HEK293 cells (Cat no:22373-1-AP)
FH Tatyana (Verified Customer) (12-04-2023)	Detects P-bodies in ICC, good signal Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human / HeLa cells DCP1A Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:22373-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 22373-1-AP

WB analysis of mouse brain using 22373-1-AP

WB analysis of mouse lung using 22373-1-AP

WB analysis of Jurkat using 22373-1-AP

IP experiment of mouse brain using 22373-1-AP

IHC staining of mouse lung using 22373-1-AP

IHC staining of mouse lung using 22373-1-AP

IF Staining of HepG2 using 22373-1-AP

IF Staining of HepG2 using 22373-1-AP

FC experiment of HeLa using 22373-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation

LncRNA HOXD-AS2 regulates miR-3681-5p/DCP1A axis to promote the progression of non-small cell lung cancer

DDX6 modulates P-body and stress granule assembly, composition, and docking

Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A

Reviews