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DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 22373-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Smad4-interacting transcriptional co-activator, Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse lung tissue, Jurkat cells, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22373-1-AP targets DCP1A in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCP1A fusion protein Ag18027 相同性解析による交差性が予測される生物種
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Calculated molecular weight 582 aa, 63 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007439
Gene Symbol DCP1A
Gene ID (NCBI) 55802
RRIDAB_2879092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9NPI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Protocols

Product Specific Protocols
WB protocol for DCP1A antibody 22373-1-APDownload protocol
IHC protocol for DCP1A antibody 22373-1-APDownload protocol
IF protocol for DCP1A antibody 22373-1-APDownload protocol
IP protocol for DCP1A antibody 22373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

FEBS Lett

Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

Authors - Dongxu Jiang
humanWB,IF

Cell Rep

DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling

Authors - Ke Zhang
mouseIF,IP

Cell Rep

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation

Authors - Nicolas Curdy
humanWB

J Thorac Dis

LncRNA HOXD-AS2 regulates miR-3681-5p/DCP1A axis to promote the progression of non-small cell lung cancer

Authors - Yuanyuan Zhang
humanIF,WB

J Cell Biol

DDX6 modulates P-body and stress granule assembly, composition, and docking

Authors - Nina Ripin
humanWB

J Proteome Res

Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A

Authors - Qihang Peng
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