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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCP1A Monoclonal antibody (60494-1-Ig)

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DCP1A Monoclonal antibody (60494-1-Ig)

    WB analysis using 60494-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60494-1-Ig (DCP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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DCP1A Monoclonal antibody

DCP1A Monoclonal Antibody for WB, ELISA
Cat No. 60494-1-Ig
Clone No.2D11E2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986, EC:3.6.1.62

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLoVo cells, rat brain tissue, HCT 116 cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, mouse brain tissue, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60494-1-Ig targets DCP1A in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DCP1A fusion protein Ag18706 相同性解析による交差性が予測される生物種
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Calculated molecular weight 582 aa, 63 kDa
Observed molecular weight70 kDa
GenBank accession numberBC007439
Gene Symbol DCP1A
Gene ID (NCBI) 55802
RRIDAB_3670263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Protocols

Product Specific Protocols
WB protocol for DCP1A antibody 60494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols