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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCP1B Polyclonal antibody

DCP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13863-1-AP

Synonyms

mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCP1B Polyclonal antibody (13863-1-AP)

    WB analysis using 13863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DCP1B Polyclonal antibody (13863-1-AP)

    WB analysis of Jurkat using 13863-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DCP1B Polyclonal antibody (13863-1-AP)

    WB analysis of human liver using 13863-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DCP1B Polyclonal antibody (13863-1-AP)

    WB analysis of HeLa using 13863-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using DCP1B Polyclonal antibody (13863-1-AP)

    WB analysis of rat spleen using 13863-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DCP1B Polyclonal antibody (13863-1-AP)

    IP experiment of HeLa using 13863-1-AP

    IP result of anti-DCP1B (IP:13863-1-AP, 4ug; Detection:13863-1-AP 1:500) with HeLa cells lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DCP1B Polyclonal antibody (13863-1-AP)

    IHC staining of human liver cancer using 13863-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DCP1B Polyclonal antibody (13863-1-AP)

    IHC staining of human liver cancer using 13863-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DCP1B Polyclonal antibody (13863-1-AP)

    IF Staining of HeLa using 13863-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCP1B antibody (13863-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DCP1B Polyclonal antibody (13863-1-AP)

    IF Staining of A549 using 13863-1-AP

    Immunofluorescent analysis of A549 cells using 13863-1-AP (DCP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, human liver tissue, Jurkat cells, rat liver tissue, rat spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13863-1-AP targets DCP1B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCP1B fusion protein Ag4845 相同性解析による交差性が予測される生物種
Full Name DCP1 decapping enzyme homolog B (S. cerevisiae)
Calculated molecular weight 618 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC043437
Gene symbol DCP1B
Gene ID (NCBI) 196513
RRIDAB_2090411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCP1B belongs to DCP1 family. DCP1B may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP.  DCP1B has 2 isoforms with the molecular mass of 56 and 68 kDa. DCP1B is associated with DM-related atherogenesis (PMID:33195466).

Protocols

Product Specific Protocols
WB protocol for DCP1B antibody 13863-1-APDownload protocol
IHC protocol for DCP1B antibody 13863-1-APDownload protocol
IF protocol for DCP1B antibody 13863-1-APDownload protocol
IP protocol for DCP1B antibody 13863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Int J Mol Med

LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication.

Authors - Yangchao Dong
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

George (Verified Customer) (08-19-2019)

Shows up well under concentration used - diffuse in unstressed tissue, forms distinct granular structures following 90 minutes 42oC heat shock. Only tested using 488 secondary antibody but showed up brightly under low power microscopy.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cortical Slice Culture