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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCP2 Polyclonal antibody (17744-1-AP)

    WB analysis using 17744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17744-1-AP (DCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DCP2 Polyclonal antibody (17744-1-AP)

    WB analysis using 17744-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 17744-1-AP (DCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DCP2 Polyclonal antibody (17744-1-AP)

    IHC staining of human ovary cancer using 17744-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17744-1-AP (DCP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DCP2 Polyclonal antibody

DCP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17744-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Nudix motif 20, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, m7GpppN-mRNA hydrolase, hDpc

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Caco-2 cells, T-47D cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17744-1-AP targets DCP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCP2 fusion protein Ag12001 相同性解析による交差性が予測される生物種
Full Name DCP2 decapping enzyme homolog (S. cerevisiae)
Calculated molecular weight385aa,44 kDa; 420aa,48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC064593
Gene Symbol DCP2
Gene ID (NCBI) 167227
RRIDAB_3085538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IU60
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DCP2 antibody 17744-1-APDownload protocol
IHC protocol for DCP2 antibody 17744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols