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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCT Monoclonal antibody

DCT Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D4

Cat no : 68114-1-Ig

Synonyms

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of A375 using 68114-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of HL-60 using 68114-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of pig skin using 68114-1-Ig

    pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse testis using 68114-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse skin using 68114-1-Ig

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DCT Monoclonal antibody (68114-1-Ig)

    IF Staining of A549 using 68114-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA375 cells, HL-60 cells, pig skin tissue, mouse skin tissue, mouse testis tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68114-1-Ig targets DCT in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DCT fusion protein Ag26165 相同性解析による交差性が予測される生物種
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight55-63 kDa
GenBank accession numberBC028311
Gene symbol DCT
Gene ID (NCBI) 1638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Protocols

Product Specific Protocols
WB protocol for DCT antibody 68114-1-IgDownload protocol
IF protocol for DCT antibody 68114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols