p150 glued Polyclonal antibody
p150 glued Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55182-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, Jurkat cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
55182-1-AP targets p150 glued in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine samples.
| Tested Reactivity | human, Canine |
| Cited Reactivity | human, mussel |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | dynactin 1 (p150, glued homolog, Drosophila) |
| Calculated molecular weight | 142 kDa |
| Observed molecular weight | 135 kDa, 150 kDa |
| GenBank accession number | NM_004082 |
| Gene symbol | DCTN1 |
| Gene ID (NCBI) | 1639 |
| RRID | AB_10837074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p150 glued antibody 55182-1-AP | Download protocol |
| IHC protocol for p150 glued antibody 55182-1-AP | Download protocol |
| IF protocol for p150 glued antibody 55182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens. | ||
