Featured Product
KD/KO Validated

DCTN6 Polyclonal antibody

Name: DCTN6 Polyclonal antibody
Brand: Proteintech
SKU: 16947-1-AP

DCTN6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16947-1-AP

Synonyms

WS 3, Protein WS-3, Protein WS 3, Dynactin subunit p27, Dynactin subunit 6

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 16947-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16947-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 16947-1-AP
IP result of anti-DCTN6 (IP:16947-1-AP, 4ug; Detection:16947-1-AP 1:500) with mouse testis tissue lysate 4800ug.
IHC staining of mouse testis using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16947-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

-/ -

Cat No. 16947-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, mouse lung tissue, mouse testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse testis tissue, human kidney tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:600-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

16947-1-AP targets DCTN6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DCTN6 fusion protein Ag10561 相同性解析による交差性が予測される生物種
Full Name	dynactin 6
Calculated molecular weight	190 aa, 21 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC013175
Gene symbol	DCTN6
Gene ID (NCBI)	10671
RRID	AB_2245654
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DCTN6 antibody 16947-1-AP	Download protocol
IHC protocol for DCTN6 antibody 16947-1-AP	Download protocol
IP protocol for DCTN6 antibody 16947-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Monkey	WB	EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. Authors - Yeh Ting-Yu TY KD Validated View Article
human	WB	Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. Authors - Florencia di Pietro View Article
human	WB	Mol Biol Cell Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles. Authors - Samantha L Cavolo View Article
mouse	WB,IF,IP	Neurobiol Aging Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains. Authors - Md Golam Sharoar KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCTN6 Polyclonal antibody

DCTN6 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 16947-1-AP

WB analysis of mouse lung using 16947-1-AP

IP experiment of mouse testis using 16947-1-AP

IHC staining of mouse testis using 16947-1-AP

IHC staining of mouse testis using 16947-1-AP

IHC staining of human kidney using 16947-1-AP

IHC staining of human kidney using 16947-1-AP

IHC staining of human spleen using 16947-1-AP

IHC staining of human spleen using 16947-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.

An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.

Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.

Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.