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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DCUN1D3 Polyclonal antibody (26356-1-AP)

    WB analysis of MCF-7 using 26356-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26356-1-AP (DCUN1D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DCUN1D3 Polyclonal antibody (26356-1-AP)

    WB analysis of A549 using 26356-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26356-1-AP (DCUN1D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DCUN1D3 Polyclonal antibody

DCUN1D3 Polyclonal Antibody for WB, ELISA
Cat No. 26356-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

44M2.4, DCN1 like protein 3, DCUN1D3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26356-1-AP targets DCUN1D3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCUN1D3 fusion protein Ag23730 相同性解析による交差性が予測される生物種
Full Name DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae)
Observed molecular weight 34 kDa
GenBank accession numberBC040442
Gene Symbol DCUN1D3
Gene ID (NCBI) 123879
RRIDAB_2880489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCUN1D3, also named as SCCRO3, is proposed to function as a tumor suppressor. DCUN1D3 antagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, DCUN1D3 may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).

Protocols

Product Specific Protocols
WB protocol for DCUN1D3 antibody 26356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols