Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of HeLa using 14810-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14810-1-AP
WB result of DCUN1D5 antibody (14810-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCUN1D5 transfected HEK-293 cells.
WB analysis of HEK-293 using 14810-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 14810-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14810-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 14810-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14810-1-AP
IP result of anti-DCUN1D5 (IP:14810-1-AP, 3ug; Detection:14810-1-AP 1:800) with HeLa cells lysate 1840ug.
IF Staining of HeLa using 14810-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14810-1-AP (DCUN1D5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DCUN1D5 Polyclonal antibody

DCUN1D5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14810-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Squamous cell carcinoma-related oncogene 5, SCCRO5, Defective in cullin neddylation protein 1-like protein 5, DCUN1 domain-containing protein 5, DCNL5

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse brain tissue, rat spleen tissue, U-937 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 2 publications below

Product Information

14810-1-AP targets DCUN1D5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DCUN1D5 fusion protein Ag6534 相同性解析による交差性が予測される生物種
Full Name	DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae)
Calculated molecular weight	28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC004169
Gene Symbol	DCUN1D5
Gene ID (NCBI)	84259
RRID	AB_2088468
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BTE7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Conjugation of Nedd8 to a cullin protein, termed neddylation, is an evolutionarily conserved process that functions to activate the cullin-RING family E3 ubiquitin ligases, leading to increased proteasomal degradation of a wide range of substrate proteins. Cellular neddylation requires the action of DCN1 (Defective in cullin neddylation protein 1), which, in humans, consists of five homologues designated as DCNL1-5. DCUN1D5 gene encodes the 28 kDa DCUN1 domain-containing protein 5 containing a C-terminal potentiating neddylation domain. The cellular function of DCUN1D5 needs to be explored, recently, a mono-ubiquitination-mediated mechanism that governs nuclear-cytoplasmic trafficking of hDCNL1, was reported to regulate hDCNL1-dependent activation of the cullin-RING E3 ubiquitin ligases in selected cellular compartments.

Protocols

Product Specific Protocols
WB protocol for DCUN1D5 antibody 14810-1-AP	Download protocol
IF protocol for DCUN1D5 antibody 14810-1-AP	Download protocol
IP protocol for DCUN1D5 antibody 14810-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Authors - Kate E Coleman View Article
human	WB,IHC	Asian Pac J Cancer Prev In vitro biological characterization of DCUN1D5 in DNA damage response. Authors - Guo Wei W View Article
human	WB,IHC	Biol Direct YY1 mediated DCUN1D5 transcriptional activation promotes triple-negative breast cancer progression by targeting FN1/PI3K/AKT pathway Authors - Yuxiang Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14810-1-AP

WB analysis of HEK-293 using 14810-1-AP

WB analysis of HEK-293 using 14810-1-AP

WB analysis of U-937 using 14810-1-AP

WB analysis of mouse brain using 14810-1-AP

WB analysis of rat spleen using 14810-1-AP

IP experiment of HeLa using 14810-1-AP

IF Staining of HeLa using 14810-1-AP