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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCX Polyclonal antibody, PBS Only

DCX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13925-1-PBS

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCX Polyclonal antibody (13925-1-AP)

    WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DCX Polyclonal antibody (13925-1-AP)

    WB analysis using 13925-1-AP (same clone as 13925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DCX Polyclonal antibody (13925-1-AP)

    IHC staining of rat brain using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DCX Polyclonal antibody (13925-1-AP)

    IHC staining of mouse brain using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DCX Polyclonal antibody (13925-1-AP)

    IHC staining of human gliomas using 13925-1-AP (same clone as 13925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using DCX Polyclonal antibody (13925-1-AP)

    IF Staining of SH-SY5Y using 13925-1-AP (same clone as 13925-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13925-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13925-1-PBS targets DCX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCX fusion protein Ag4945 相同性解析による交差性が予測される生物種
Full Name doublecortin
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC027925
Gene symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.