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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using DCX Recombinant antibody (84595-4-RR)

    WB analysis of fetal human brain using 84595-4-RR

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using DCX Recombinant antibody (84595-4-RR)

    WB analysis of SH-SY5Y using 84595-4-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DCX Recombinant antibody (84595-4-RR)

    IHC staining of rat brain using 84595-4-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

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DCX Recombinant antibody

DCX Recombinant Antibody for WB, IHC, ELISA
Cat No. 84595-4-RR
Clone No.241985H1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, fetal human brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84595-4-RR targets DCX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DCX fusion protein Ag4945 相同性解析による交差性が予測される生物種
Full Name doublecortin
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

Protocols

Product Specific Protocols
WB protocol for DCX antibody 84595-4-RRDownload protocol
IHC protocol for DCX antibody 84595-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols