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  • Featured Product
  • KD/KO Validated

DDA1 Polyclonal antibody

DDA1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14995-1-AP

Synonyms

Placenta cross-immune reaction antigen 1, PCIA-1, PCIA1, DET1- and DDB1-associated protein 1, C19orf58

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDA1 Polyclonal antibody (14995-1-AP)

    WB analysis using 14995-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DDA1 Polyclonal antibody (14995-1-AP)

    WB analysis of mouse liver using 14995-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using DDA1 Polyclonal antibody (14995-1-AP)

    IP experiment of HepG2 using 14995-1-AP

    IP result of anti-DDA1 (IP:14995-1-AP, 4ug; Detection:14995-1-AP 1:500) with HepG2 cells lysate 1080 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDA1 Polyclonal antibody (14995-1-AP)

    IF Staining of HepG2 using 14995-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDA1 antibody (14995-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using DDA1 Polyclonal antibody (14995-1-AP)

    FC experiment of HepG2 using 14995-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDA1 Polyclonal antibody (14995-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14995-1-AP targets DDA1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDA1 fusion protein Ag6981 相同性解析による交差性が予測される生物種
Full Name DET1 and DDB1 associated 1
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC000615
Gene symbol DDA1
Gene ID (NCBI) 79016
RRIDAB_10859095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).

Protocols

Product Specific Protocols
WB protocol for DDA1 antibody 14995-1-APDownload protocol
IF protocol for DDA1 antibody 14995-1-APDownload protocol
IP protocol for DDA1 antibody 14995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRL4AMBRA1 is a master regulator of D-type cyclins.

Authors - Daniele Simoneschi
View Article
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han
View Article
humanWB

Nat Chem Biol

Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.

Authors - Dirksen E Bussiere
  • KD Validated
View Article
mouseIP, WB

Curr Biol

Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Authors - Luca Rizzini
View Article
humanIHC,IF

EMBO Mol Med

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

Authors - Lucía Zhu
View Article
humanWB

Cell Rep

Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.

Authors - Tabitha C Ting
View Article