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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDAH1 Monoclonal antibody

DDAH1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A6

Cat no : 68823-1-Ig

Synonyms

DDAH, DDAH 1, DDAH1, DDAHI, Dimethylargininase 1, FLJ21264, FLJ25539

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDAH1 Monoclonal antibody (68823-1-Ig)

    WB analysis using 68823-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DDAH1 Monoclonal antibody (68823-1-Ig)

    WB analysis using 68823-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCalu-3 cells, SH-SY5Y cells, HT-29 cells, SK-N-SH cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68823-1-Ig targets DDAH1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDAH1 fusion protein Ag32840 相同性解析による交差性が予測される生物種
Full Name dimethylarginine dimethylaminohydrolase 1
Calculated molecular weight31kd
Observed molecular weight35 kDa
GenBank accession numberNM_012137
Gene symbol DDAH1
Gene ID (NCBI) 23576
RRIDAB_3670434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.

Protocols

Product Specific Protocols
WB protocol for DDAH1 antibody 68823-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols