Validation Data Gallery
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Tested Applications
| Positive WB detected in | rabbit brain tissue, mouse retina tissue, rat colon tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68154-1-Ig targets DDAH2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit samples.
| Tested Reactivity | Human, Rat, Mouse, Rabbit |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DDAH2 fusion protein Ag7127 相同性解析による交差性が予測される生物種 |
| Full Name | dimethylarginine dimethylaminohydrolase 2 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC001435 |
| Gene Symbol | DDAH2 |
| Gene ID (NCBI) | 23564 |
| RRID | AB_2923677 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95865 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDAH2 antibody 68154-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |