• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

DDB1 Polyclonal antibody, PBS Only

DDB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11380-1-PBS

Synonyms

UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis using 11380-1-AP (same clone as 11380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of mouse testis using 11380-1-AP (same clone as 11380-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human kidney using 11380-1-AP (same clone as 11380-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human placenta using 11380-1-AP (same clone as 11380-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of rat testis using 11380-1-AP (same clone as 11380-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human brain using 11380-1-AP (same clone as 11380-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using DDB1 Polyclonal antibody (11380-1-AP)

    IP experiment of Jurkat using 11380-1-AP (same clone as 11380-1-PBS)

    IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11380-1-AP (DDB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11380-1-PBS targets DDB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDB1 fusion protein Ag1901 相同性解析による交差性が予測される生物種
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated molecular weight 1140 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC011686
Gene symbol DDB1
Gene ID (NCBI) 1642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.