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  • Featured Product
  • KD/KO Validated

DDB1 Polyclonal antibody, PBS Only

DDB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11380-1-PBS

Synonyms

UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis using 11380-1-AP (same clone as 11380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of mouse testis using 11380-1-AP (same clone as 11380-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human kidney using 11380-1-AP (same clone as 11380-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human placenta using 11380-1-AP (same clone as 11380-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of human brain using 11380-1-AP (same clone as 11380-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using DDB1 Polyclonal antibody (11380-1-AP)

    WB analysis of rat testis using 11380-1-AP (same clone as 11380-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using DDB1 Polyclonal antibody (11380-1-AP)

    IP experiment of Jurkat using 11380-1-AP (same clone as 11380-1-PBS)

    IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11380-1-AP (DDB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Polyclonal antibody (11380-1-AP)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11380-1-PBS targets DDB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDB1 fusion protein Ag1901 相同性解析による交差性が予測される生物種
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated molecular weight 1140 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC011686
Gene symbol DDB1
Gene ID (NCBI) 1642
RRIDAB_2088808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.