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  • Featured Product
  • KD/KO Validated

DDB1 Monoclonal antibody, PBS Only

DDB1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F6C6

Cat no : 66010-1-PBS

Synonyms

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis using 66010-1-Ig (same clone as 66010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of A549 using 66010-1-Ig (same clone as 66010-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    N/A incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    IF Staining of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunofluorescent analysis of fixed MCF-7 cells using 66010-1-Ig (DDB1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66010-1-PBS targets DDB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DDB1 fusion protein Ag17903 相同性解析による交差性が予測される生物種
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated molecular weight 1140 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC011686
Gene symbol DDB1
Gene ID (NCBI) 1642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.