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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDB2 Polyclonal antibody, PBS Only

DDB2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10431-1-PBS

Synonyms

UV-DDB 2, UV-damaged DNA-binding protein 2, UV DDB 2, DNA damage-binding protein 2, DDBB

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDB2 Polyclonal antibody (10431-1-AP)

    WB analysis of HeLa using 10431-1-AP (same clone as 10431-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10431-1-AP (DDB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10431-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DDB2 Polyclonal antibody (10431-1-AP)

    WB analysis of A431 using 10431-1-AP (same clone as 10431-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10431-1-AP (DDB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10431-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDB2 Polyclonal antibody (10431-1-AP)

    IF Staining of HeLa using 10431-1-AP (same clone as 10431-1-PBS)

    Immunofluorescent analysis of HeLa cells using 10431-1-AP (DDB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10431-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10431-1-PBS targets DDB2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDB2 fusion protein Ag0691 相同性解析による交差性が予測される生物種
Full Name damage-specific DNA binding protein 2, 48kDa
Calculated molecular weight 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC000093
Gene symbol DDB2
Gene ID (NCBI) 1643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDB2, also named as DNA damage-binding protein 2 or UV-damaged DNA-binding protein 2, is a 427 amino acid protein, which contains seven WD repeats and belongs to the WD repeat DDB2/WDR76 family. DDB2 is ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. DDB2 localizes in the nucleus and is required for DNA repair. DDB2 regulates negatively the constitutive expression of the SOD2 gene in breast cancer cells and exerts, at least in part, a control of breast cancer cell growth. DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer.