DDIT4L Polyclonal antibody
DDIT4L Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28490-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse ovary tissue |
Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28490-1-AP targets DDIT4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DDIT4L fusion protein Ag29616 相同性解析による交差性が予測される生物種 |
Full Name | DNA-damage-inducible transcript 4-like |
Calculated molecular weight | 193 aa, 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC013592 |
Gene symbol | DDIT4L |
Gene ID (NCBI) | 115265 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.
Protocols
Product Specific Protocols | |
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WB protocol for DDIT4L antibody 28490-1-AP | Download protocol |
IHC protocol for DDIT4L antibody 28490-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |