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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDIT4L Polyclonal antibody

DDIT4L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28490-1-AP

Synonyms

RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDIT4L Polyclonal antibody (28490-1-AP)

    WB analysis using 28490-1-AP

    Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 28490-1-AP (DDIT4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using DDIT4L Polyclonal antibody (28490-1-AP)

    IHC staining of mouse ovary using 28490-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using DDIT4L Polyclonal antibody (28490-1-AP)

    IHC staining of mouse ovary using 28490-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse ovary tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28490-1-AP targets DDIT4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDIT4L fusion protein Ag29616 相同性解析による交差性が予測される生物種
Full Name DNA-damage-inducible transcript 4-like
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC013592
Gene symbol DDIT4L
Gene ID (NCBI) 115265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.

Protocols

Product Specific Protocols
WB protocol for DDIT4L antibody 28490-1-APDownload protocol
IHC protocol for DDIT4L antibody 28490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols