DDR1 Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP01290-4, Humam DDR1 Recombinant Matched Antibody Pair - PBS only. 84438-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1675. 84438-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 4 mM L-glutamine, 4500mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human DDR1, and measured 19.81 ng/mL

The mean DDR1 concentration was determined to be 9.28 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

Serum of four individual healthy human donors was measured. The human DDR1 concentration of detected samples was determined to be 13.63 ng/mL with a range of 9.29 - 18.03 ng/mL.

Cytometric bead array standard curve of MP01290-1, DDR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84438-2-PBS. Detection antibody: 84438-3-PBS. Standard: Eg1675. Range: 0.313-40 ng/mL.

Cytometric bead array standard curve of MP01290-3, DDR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84438-4-PBS. Detection antibody: 84438-3-PBS. Standard: Eg1675. Range: 0.313-40 ng/mL.