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- KD/KO Validated
DDX1 Polyclonal antibody
DDX1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11357-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, PC-3 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human stomach tissue, human lymphoma tissue, human ovary tumor tissue, mouse brain tissue, mouse lung tissue, mouse skin tissue, rat brain tissue, rat lung tissue, rat skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 16 publications below |
| IHC | See 3 publications below |
| IF | See 6 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
| RIP | See 2 publications below |
Product Information
11357-1-AP targets DDX1 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDX1 fusion protein Ag1887 相同性解析による交差性が予測される生物種 |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 |
| Calculated molecular weight | 740 aa, 82 kDa |
| Observed molecular weight | 70-82 kDa |
| GenBank accession number | BC012132 |
| Gene symbol | DDX1 |
| Gene ID (NCBI) | 1653 |
| RRID | AB_2092222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX1 antibody 11357-1-AP | Download protocol |
| IHC protocol for DDX1 antibody 11357-1-AP | Download protocol |
| IP protocol for DDX1 antibody 11357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.
| ||
Cell Host Microbe RNase L Activates the NLRP3 Inflammasome during Viral Infections.
| ||
Cell Syst BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ilham (Verified Customer) (08-06-2024) | It works very well - the AB was diluted 1:2000 in 5% BSA -chemiluminescence blots using the ChemiDoc Imaging System.
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