• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX18 Polyclonal antibody (28502-1-AP)

    WB analysis using 28502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28502-1-AP (DDX18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using DDX18 Polyclonal antibody (28502-1-AP)

    IP experiment of HEK-293T using 28502-1-AP

    IP result of anti-DDX18 (IP:28502-1-AP, 4ug; Detection:28502-1-AP 1:1500) with HEK-293T cells lysate 1560 ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DDX18 Polyclonal antibody (28502-1-AP)

    IF Staining of HEK-293 using 28502-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX18 antibody (28502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

DDX18 Polyclonal antibody

DDX18 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 28502-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293T cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28502-1-AP targets DDX18 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX18 fusion protein Ag27457 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Calculated molecular weight 75 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC001238
Gene Symbol DDX18
Gene ID (NCBI) 8886
RRIDAB_2881158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDX18 antibody 28502-1-APDownload protocol
IF protocol for DDX18 antibody 28502-1-APDownload protocol
IP protocol for DDX18 antibody 28502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols