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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of K-562 using 18285-1-AP (same clone as 18285-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of HEK-293 using 18285-1-AP (same clone as 18285-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of NIH/3T3 using 18285-1-AP (same clone as 18285-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of HeLa using 18285-1-AP (same clone as 18285-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18285-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using DDX19B Polyclonal antibody (18285-1-AP)

    IP experiment of K-562 using 18285-1-AP (same clone as 18285-1-PBS)

    IP result of anti-DDX19B (IP:18285-1-AP, 4ug; Detection:18285-1-AP 1:500) with K-562 cells lysate 4000ug. This data was developed using the same antibody clone with 18285-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18285-1-PBS targets DDX19B in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX19B fusion protein Ag13165 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19B
Calculated molecular weight479aa,54 kDa; 370aa,42 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010008
Gene Symbol DDX19B
Gene ID (NCBI) 11269
RRIDAB_10597088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMR2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.