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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis using 11324-1-AP (same clone as 11324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis of Jurkat using 11324-1-AP (same clone as 11324-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using DDX20 Polyclonal antibody (11324-1-AP)

    WB analysis of mouse testis using 11324-1-AP (same clone as 11324-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11324-1-AP (DDX20 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DDX20 Polyclonal antibody (11324-1-AP)

    IP experiment of HeLa using 11324-1-AP (same clone as 11324-1-PBS)

    IP result of anti-DDX20 (IP:11324-1-AP, 4ug; Detection:11324-1-AP 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human breast cancer using 11324-1-AP (same clone as 11324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human breast cancer using 11324-1-AP (same clone as 11324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11324-1-AP (DDX20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDX20 Polyclonal antibody (11324-1-AP)

    IHC staining of human colon cancer using 11324-1-AP (same clone as 11324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11324-1-AP (DDX20 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX20 Polyclonal antibody (11324-1-AP)

    IF Staining of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using DDX20 antibody 11324-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX20 Polyclonal antibody (11324-1-AP)

    IF Staining of HeLa using 11324-1-AP (same clone as 11324-1-PBS)

    Immunofluorescent analysis of Hela cells, using DDX20 antibody 11324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using DDX20 Polyclonal antibody (11324-1-AP)

    FC experiment of HepG2 using 11324-1-AP (same clone as 11324-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX20 (11324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11324-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DDX20 Polyclonal antibody, PBS Only

DDX20 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11324-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11324-1-PBS targets DDX20 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX20 fusion protein Ag1863 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Calculated molecular weight 824 aa, 92 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC011556
Gene Symbol DDX20
Gene ID (NCBI) 11218
RRIDAB_2092401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHI6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.