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  • Featured Product
  • KD/KO Validated

DDX21 Polyclonal antibody

DDX21 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10528-1-AP

Synonyms

Gu-alpha, GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX21 Polyclonal antibody (10528-1-AP)

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DDX21 Polyclonal antibody (10528-1-AP)

    WB analysis of HeLa using 10528-1-AP

    WB result of DDX21 antibody (10528-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DDX21 Polyclonal antibody (10528-1-AP)

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DDX21 Polyclonal antibody (10528-1-AP)

    WB analysis using 10528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using DDX21 Polyclonal antibody (10528-1-AP)

    IP experiment of COLO 320 using 10528-1-AP

    IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using DDX21 Polyclonal antibody (10528-1-AP)

    IP experiment of COLO 320 using 10528-1-AP

    IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:15000) with COLO 320 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDX21 Polyclonal antibody (10528-1-AP)

    IHC staining of human colon cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDX21 Polyclonal antibody (10528-1-AP)

    IHC staining of human colon cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX21 Polyclonal antibody (10528-1-AP)

    IHC staining of human breast cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX21 Polyclonal antibody (10528-1-AP)

    IHC staining of human breast cancer using 10528-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX21 Polyclonal antibody (10528-1-AP)

    IF Staining of HeLa using 10528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX21 Polyclonal antibody (10528-1-AP)

    IF Staining of HeLa using 10528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using DDX21 Polyclonal antibody (10528-1-AP)

    FC experiment of HepG2 using 10528-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX21 (10528-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells, PC-3 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10528-1-AP targets DDX21 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX21 fusion protein Ag0804 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC008071
Gene symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2092705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. Catalog# 10528-1-AP is a rabbit polyclonal antibody raised against N-terminal of human DDX21.

Protocols

Product Specific Protocols
WB protocol for DDX21 antibody 10528-1-APDownload protocol
IHC protocol for DDX21 antibody 10528-1-APDownload protocol
IF protocol for DDX21 antibody 10528-1-APDownload protocol
IP protocol for DDX21 antibody 10528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Science

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Authors - Man Wu
View Article
humanWB,IF,chIP

Cell

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Authors - Yu-Hang Xing
  • KO Validated
View Article
humanWB,IHC,IF

Mol Cell

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Authors - Dae-Seok Kim
View Article
mouseWB

Dev Cell

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Authors - Dingfeng Zou
View Article
humanIP

Dev Cell

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Authors - Jeanette A Johansson
View Article
humanWB,IF

Cancer Res

The roles of RNA helicases in DNA damage repair and tumorigenesis reveal precision therapeutic strategies.

Authors - Jinru Xie
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alexandra (Verified Customer) (09-04-2023)

Very good signal. I would recommend a much higher dilution than 1:200.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: P19 derived neurons
DDX21 Antibody Immunofluorescence validation (1:200 dilution) in P19 derived neurons (Cat no:10528-1-AP)
FH

Wael (Verified Customer) (12-13-2022)

Hek293 fractionated to nuclear and cytoplasmic fractions, DDX21 antibody detects strong band at the expected size of DDX21 and showing nuclear localization as expected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
DDX21 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:10528-1-AP)