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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX21 Recombinant antibody

DDX21 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E7

Cat no : 80932-1-RR

Synonyms

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX21 Recombinant antibody (80932-1-RR)

    WB analysis using 80932-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX21 Recombinant antibody (80932-1-RR)

    IF Staining of HepG2 using 80932-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX21 Recombinant antibody (80932-1-RR)

    IF Staining of HepG2 using 80932-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 80932-1-RR

    Affinity and Kinetic Characterization of 80932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, THP-1 cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80932-1-RR targets DDX21 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX21 fusion protein Ag0804 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC008071
Gene symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2918920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.

Protocols

Product Specific Protocols
WB protocol for DDX21 antibody 80932-1-RRDownload protocol
IF protocol for DDX21 antibody 80932-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols