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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX24 Recombinant antibody

DDX24 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241145G10

Cat no : 84103-6-RR

Synonyms

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX24 Recombinant antibody (84103-6-RR)

    WB analysis using 84103-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX24 Recombinant antibody (84103-6-RR)

    IF Staining of HeLa using 84103-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U-2 OS using DDX24 Recombinant antibody (84103-6-RR)

    FC experiment of U-2 OS using 84103-6-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inY79 cells, HeLa cells, COLO 320 cells, A549 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84103-6-RR targets DDX24 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX24 fusion protein Ag8407 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Calculated molecular weight 96 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC008847
Gene symbol DDX24
Gene ID (NCBI) 57062
RRIDAB_3671669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

Protocols

Product Specific Protocols
WB protocol for DDX24 antibody 84103-6-RRDownload protocol
IF protocol for DDX24 antibody 84103-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols