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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX27 Polyclonal antibody

DDX27 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17087-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDX27 Polyclonal antibody (17087-1-AP)

    WB analysis of HeLa using 17087-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DDX27 Polyclonal antibody (17087-1-AP)

    WB analysis of mouse liver using 17087-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DDX27 Polyclonal antibody (17087-1-AP)

    WB analysis of NIH/3T3 using 17087-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX27 Polyclonal antibody (17087-1-AP)

    IF Staining of HeLa using 17087-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17087-1-AP (DDX27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DDX27 Polyclonal antibody (17087-1-AP)

    IF Staining of HEK-293 using 17087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX27 antibody (17087-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, NIH/3T3 cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17087-1-AP targets DDX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX27 fusion protein Ag9426 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
Calculated molecular weight 796 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC016060
Gene symbol DDX27
Gene ID (NCBI) 55661
RRIDAB_2092569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

Protocols

Product Specific Protocols
WB protocol for DDX27 antibody 17087-1-APDownload protocol
IF protocol for DDX27 antibody 17087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.

Authors - Francesca Rossi
View Article
humanWB

Onco Targets Ther

DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.

Authors - Chunxing Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wael (Verified Customer) (05-24-2023)

we detected the relative abundance of DDX27 in both the Nuclear and cytoplasmic fractions

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
DDX27 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17087-1-AP)