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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX3 Monoclonal antibody

DDX3 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D3A1

Cat no : 67915-1-Ig

Synonyms

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX3 Monoclonal antibody (67915-1-Ig)

    WB analysis using 67915-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67915-1-Ig (DDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using DDX3 Monoclonal antibody (67915-1-Ig)

    WB analysis of HepG2 using 67915-1-Ig

    WB result of DDX3 antibody (67915-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX3 Monoclonal antibody (67915-1-Ig)

    IHC staining of human breast cancer using 67915-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX3 Monoclonal antibody (67915-1-Ig)

    IHC staining of human breast cancer using 67915-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67915-1-Ig (DDX3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using DDX3 Monoclonal antibody (67915-1-Ig)

    FC experiment of HepG2 using 67915-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human DDX3 (67915-1-Ig, Clone:1D3A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67915-1-Ig targets DDX3 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DDX3 fusion protein Ag29972 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated molecular weight 73 kDa
Observed molecular weight71-73 kDa
GenBank accession numberBC011819
Gene symbol DDX3
Gene ID (NCBI) 1654
RRIDAB_2918669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Protocols

Product Specific Protocols
WB protocol for DDX3 antibody 67915-1-IgDownload protocol
IHC protocol for DDX3 antibody 67915-1-IgDownload protocol
FC protocol for DDX3 antibody 67915-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities

Authors - Nina Đukić
View Article
ratIF

CNS Neurosci Ther

A novel stroke rehabilitation strategy and underlying stress granule regulations through inhibition of NLRP3 inflammasome activation

Authors - Qingzhu Wang
View Article