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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX3 Recombinant antibody (81903-1-RR)

    WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DDX3 Recombinant antibody (81903-1-RR)

    WB analysis of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    WB result of DDX3 antibody (81903-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX3 Recombinant antibody (81903-1-RR)

    IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using DDX3 Recombinant antibody (81903-1-RR)

    FC experiment of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDX3 Recombinant antibody (81903-1-RR, Clone:5C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

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DDX3 Recombinant antibody, PBS Only

DDX3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81903-1-PBS
Clone No.5C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81903-1-PBS targets DDX3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX3 fusion protein Ag1614 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated molecular weight 73 kDa
Observed molecular weight75 kDa
GenBank accession numberBC011819
Gene Symbol DDX3
Gene ID (NCBI) 1654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00571
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.