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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX39A Recombinant antibody (83083-5-RR)

    WB analysis using 83083-5-RR (same clone as 83083-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DDX39A Recombinant antibody (83083-5-RR)

    WB analysis using 83083-5-RR (same clone as 83083-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DDX39A Recombinant antibody (83083-5-RR)

    WB analysis of HEK-293 using 83083-5-RR (same clone as 83083-5-PBS)

    WB result of DDX39A antibody (83083-5-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX39A transfected HEK-293 cells. This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DDX39A Recombinant antibody (83083-5-RR)

    IHC staining of mouse kidney using 83083-5-RR (same clone as 83083-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83083-5-RR (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX39A Recombinant antibody (83083-5-RR)

    IF Staining of HepG2 using 83083-5-RR (same clone as 83083-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-5-RR, Clone: 230375F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using DDX39A Recombinant antibody (83083-5-RR)

    FC experiment of Jurkat using 83083-5-RR (same clone as 83083-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human DDX39A (83083-5-RR, Clone:230375F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83083-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83083-5-RR

    Affinity and Kinetic Characterization of 83083-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83083-5-RR against Human DDX39A were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

DDX39A Recombinant antibody, PBS Only

DDX39A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83083-5-PBS
Clone No.230375F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83083-5-PBS targets DDX39A in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX39A fusion protein Ag2311 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
Calculated molecular weight 427 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC032128
Gene Symbol DDX39
Gene ID (NCBI) 10212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.