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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17545-1-AP

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX4,VASA Polyclonal antibody (17545-1-AP)

    WB analysis using 17545-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17545-1-AP (DDX4,VASA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using DDX4,VASA Polyclonal antibody (17545-1-AP)

    WB analysis of rat testis using 17545-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17545-1-AP (DDX4,VASA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4,VASA Polyclonal antibody (17545-1-AP)

    IHC staining of mouse testis using 17545-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17545-1-AP (DDX4,VASA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4,VASA Polyclonal antibody (17545-1-AP)

    IHC staining of mouse testis using 17545-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17545-1-AP (DDX4,VASA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17545-1-AP is DDX4,VASA Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX4,VASA fusion protein Ag11647 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Calculated molecular weight690aa,76 kDa; 724aa,79 kDa
Observed molecular weight79 kDa
GenBank accession numberBC047455
Gene symbol DDX4
Gene ID (NCBI) 54514
RRIDAB_2918053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Protocols

Product Specific Protocols
WB protocol for DDX4,VASA antibody 17545-1-APDownload protocol
IHC protocol for DDX4,VASA antibody 17545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Sci Rep

FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells.

Authors - Larissa Zarate-Garcia
View Article