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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX4,VASA Monoclonal antibody

DDX4,VASA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F12C3

Cat no : 67147-2-Ig

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    WB analysis of SKOV-3 using 67147-2-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    WB analysis of rat testis using 67147-2-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    WB analysis of JAR using 67147-2-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    WB analysis of NCCIT using 67147-2-Ig

    NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    IHC staining of mouse testis using 67147-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DDX4,VASA Monoclonal antibody (67147-2-Ig)

    IF Staining of mouse testis using 67147-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (67147-2-Ig, Clone: 1F12C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inSKOV-3 cells, JAR cells, rat testis tissue, NCCIT cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

67147-2-Ig targets DDX4,VASA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX4,VASA fusion protein Ag11630 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Calculated molecular weight690aa,76 kDa; 724aa,79 kDa
Observed molecular weight79 kDa
GenBank accession numberBC047455
Gene symbol DDX4
Gene ID (NCBI) 54514
RRIDAB_2882446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Protocols

Product Specific Protocols
WB protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
IHC protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
IF protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Reproduction

NR2C2 of macrophages promotes inflammation via NF-κB in LPS-induced orchitis in mice

Authors - Ziqian Min
View Article
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
View Article
ratIF

Reprod Sci

Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy

Authors - Qianwen Ma
View Article