• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX41 Polyclonal antibody

DDX41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27500-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX41, EC:3.6.4.13, DEAD box protein 41, ABS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using DDX41 Polyclonal antibody (27500-1-AP)

    WB analysis of MCF-7 using 27500-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DDX41 Polyclonal antibody (27500-1-AP)

    WB analysis of MCF-7 using 27500-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using DDX41 Polyclonal antibody (27500-1-AP)

    WB analysis of RAW 264.7 using 27500-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DDX41 Polyclonal antibody (27500-1-AP)

    IHC staining of human ovary cancer using 27500-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27500-1-AP (DDX41 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inMCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:750-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27500-1-AP targets DDX41 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX41 fusion protein Ag26593 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC015476
Gene symbol DDX41
Gene ID (NCBI) 51428
RRIDAB_2880891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.

Protocols

Product Specific Protocols
WB protocol for DDX41 antibody 27500-1-APDownload protocol
IHC protocol for DDX41 antibody 27500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Transl Med

MicroRNA-218-5p-Ddx41 axis restrains microglia-mediated neuroinflammation through downregulating type I interferon response in a mouse model of Parkinson's disease

Authors - Danlei Wang
View Article