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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX42 Polyclonal antibody (31211-1-AP)

    WB analysis using 31211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31211-1-AP (DDX42 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX42 Polyclonal antibody (31211-1-AP)

    IHC staining of mouse testis using 31211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31211-1-AP (DDX42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX42 Polyclonal antibody (31211-1-AP)

    IF Staining of HeLa using 31211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX42 antibody (31211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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DDX42 Polyclonal antibody

DDX42 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31211-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31211-1-AP targets DDX42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX42 fusion protein Ag34762 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Calculated molecular weight 103 kDa
Observed molecular weight130 kDa
GenBank accession numberBC015505
Gene Symbol DDX42
Gene ID (NCBI) 11325
RRIDAB_3669903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86XP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42) is a member of the DEAD box RNA helicase superfamily. A C-terminal portion of DDX42 interacts with the pro-apoptotic factor, apoptosis-stimulating protein of p53 protein 2 (ASPP2), thereby inhibiting its action (PMID: 30834153).

Protocols

Product Specific Protocols
WB protocol for DDX42 antibody 31211-1-APDownload protocol
IHC protocol for DDX42 antibody 31211-1-APDownload protocol
IF protocol for DDX42 antibody 31211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols