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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX43 Monoclonal antibody, PBS Only

DDX43 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B3B2

Cat no : 68454-1-PBS

Synonyms

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX43 Monoclonal antibody (68454-1-Ig)

    WB analysis using 68454-1-Ig (same clone as 68454-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DDX43 Monoclonal antibody (68454-1-Ig)

    WB analysis using 68454-1-Ig (same clone as 68454-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DDX43 Monoclonal antibody (68454-1-Ig)

    WB analysis using 68454-1-Ig (same clone as 68454-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68454-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68454-1-PBS targets DDX43 in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Rabbit samples.

Tested Reactivity Human, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX43 fusion protein Ag11484 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
Calculated molecular weight 648 aa, 73 kDa
Observed molecular weight 68-73 kDa
GenBank accession numberBC066938
Gene symbol DDX43
Gene ID (NCBI) 55510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.