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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using DDX5 Polyclonal antibody (10804-1-AP)

    WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DDX5 Polyclonal antibody (10804-1-AP)

    WB analysis of HeLa using 10804-1-AP (same clone as 10804-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5, antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DDX5 Polyclonal antibody (10804-1-AP)

    WB analysis of A431 using 10804-1-AP (same clone as 10804-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX5 Polyclonal antibody (10804-1-AP)

    IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DDX5 Polyclonal antibody (10804-1-AP)

    IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DDX5 Polyclonal antibody, PBS Only

DDX5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10804-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10804-1-PBS targets DDX5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX5 fusion protein Ag1237 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Calculated molecular weight 69 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC016027
Gene Symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2292757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17844
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd