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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX5 Polyclonal antibody

DDX5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26385-1-AP

Synonyms

DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DDX5 Polyclonal antibody (26385-1-AP)

    WB analysis of HepG2 using 26385-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DDX5 Polyclonal antibody (26385-1-AP)

    WB analysis of HeLa using 26385-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DDX5 Polyclonal antibody (26385-1-AP)

    IP experiment of HeLa using 26385-1-AP

    IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DDX5 Polyclonal antibody (26385-1-AP)

    IHC staining of mouse liver using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DDX5 Polyclonal antibody (26385-1-AP)

    IHC staining of mouse liver using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using DDX5 Polyclonal antibody (26385-1-AP)

    IHC staining of rat kidney using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using DDX5 Polyclonal antibody (26385-1-AP)

    IHC staining of rat kidney using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX5 Polyclonal antibody (26385-1-AP)

    IF Staining of HepG2 using 26385-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26385-1-AP targets DDX5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX5 fusion protein Ag24821 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Observed molecular weight 69 kDa
GenBank accession numberNM_004396
Gene symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2880498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

Protocols

Product Specific Protocols
WB protocol for DDX5 antibody 26385-1-APDownload protocol
IHC protocol for DDX5 antibody 26385-1-APDownload protocol
IF protocol for DDX5 antibody 26385-1-APDownload protocol
IP protocol for DDX5 antibody 26385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Med Chem

Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo

Authors - Xuwen Chen
View Article