DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D3D2

Cat no : 67025-1-Ig

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68


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Tested Applications

Positive WB detected inNCI-H1299 cells, A431 cells, HSC-T6 cells, HeLa cells, HEK-293 cell, HepG2 cells, HT-29 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67025-1-Ig targets DDX5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX5 fusion protein Ag24821 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Observed molecular weight 69 kDa
GenBank accession numberNM_004396
Gene symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2882340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

Protocols

Product Specific Protocols
WB protocol for DDX5 antibody 67025-1-IgDownload protocol
IHC protocol for DDX5 antibody 67025-1-IgDownload protocol
IF protocol for DDX5 antibody 67025-1-IgDownload protocol
IP protocol for DDX5 antibody 67025-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Theranostics

LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis.

Authors - Yansheng Li
humanWB,IP

Cancer Biol Ther

VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma

Authors - Shuo Yang
human,mouse,bovineWB,IF,CoIP

bioRxiv

Maf-family bZIP transcription factor NRL interacts with RNA-binding proteins and R-loops in retinal photoreceptors

Authors - Ximena Corso-Díaz
humanWB

Nucleic Acids Res

N 2-Alkyl-dG lesions elicit R-loop accumulation in the genome

Authors - Yinan Wang
humanWB,IP

Virol J

DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection

Authors - Mélanie Messmer
humanWB

J Am Chem Soc

Polymerase η Recruits DHX9 Helicase to Promote Replication across Guanine Quadruplex Structures

Authors - Feng Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Roy (Verified Customer) (06-15-2024)

Specific band detected for DDX5 from HeLa cells extract (1/1000 dilution, Overnight incubation at 4°C).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa